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UF Health University of Florida
The Herbert Wertheim UF Scripps Institute
for Biomedical Innovation & Technology
Cores & Resources Overview

Genetic Perturbation Screening
Gene knockdown and overexpression assistance for researchers

The Genetic Perturbation Screening core at UF Scripps provides gene silencing and overexpression services for researchers.

Our aim is to provide researchers with guidance in planning and help with executing gene expression modulation experiments. We currently use the technology based on shRNA for gene knock-down and cDNA libraries for overexpression studies. We are equipped with genome-wide retroviral shRNA libraries for both human and mouse genomes, Mammalian Gene Collection (MGC) cDNA library, as well as smaller focused sets targeting selected gene families. We serve as your GPS – guiding your next scientific discovery.
For more information and pricing, contact Robert Witwicki.

Libraries

  • shRNA Whole Genome Human Library (103826clones)
  • shRNA Whole Genome Mouse Library (86052 clones)
  • shRNA Epigenetic Human Library (4185 clones)
  • shRNA Kinase Human Library (4589 clones)
  • shRNA Phosphatase Human Library (1215 clones)
  • shRNA Epigenetic Mouse Library (2059 clones)
  • MGC Expression Ready cDNA Library (16617 clones)
  • CCSB Human ORFeome v1.1 Library (8269 clones)

Please inquire about potential future capabilities, such as CRISPR and lentivirus-based libraries, and about our annotation files.

Services

We offer shRNA and cDNA clones in the form of bacterial strains, isolated plasmid DNA, or packaged viruses in both single tube and 96-well formats. Our lentivirus packaging services are based on non-adherent protocols, which makes them highly scalable.

The core is equipped with PerkinElmer EnVision multimode plate reader for high-throughput readout of various screening assays based on absorbance, fluorescence, and luminescence. We also have access to a Biomek i7 Hybrid automated workstation, which enables high throughput pipetting automation for a variety of liquid handling needs.

Given that specifics of the projects performed by our core differ widely, we encourage contacting us to get the most accurate price estimate. For long term projects charges will be based on an hourly basis as this should reduce the final cost for the investigator.

Contacts